3.1 Tissue temperature increases with light duty cycle and pulse duration during patterned light stimulation (procedure 1)
We first evaluated the modification of tissue temperature when fifty blue light pulses were delivered to OB slices following the stimulation patterns described in table 1. The temperature of the tissue gradually increased over the fifty pulses for all patterns (Figure 1, inset). The higher the power, the duty cycle, and pulse duration, the more pronounced the temperature increase (Figure 1). Interestingly, temperature change did not appear to be correlated with the frequency of the stimulation within each duty cycle. At 13mW, most of the patterns induced a temperature increase that overcame 0.1 °C, a thermal condition that has been previously associated with modifications in neuronal activity (Ait Ouares et al. , 2019; Owen et al. , 2019).