3.1 Tissue temperature increases with light duty cycle and
pulse duration during patterned light stimulation (procedure 1)
We first evaluated the modification of tissue temperature when fifty
blue light pulses were delivered to OB slices following the stimulation
patterns described in table 1. The temperature of the tissue gradually
increased over the fifty pulses for all patterns (Figure 1, inset). The
higher the power, the duty cycle, and pulse duration, the more
pronounced the temperature increase (Figure 1). Interestingly,
temperature change did not appear to be correlated with the frequency of
the stimulation within each duty cycle. At 13mW, most of the patterns
induced a temperature increase that overcame 0.1 °C, a thermal condition
that has been previously associated with modifications in neuronal
activity (Ait Ouares et al. , 2019; Owen et al. , 2019).