Procedure 1: determining the profile of temperature modification
produced by light patterns
The thermal modifications produced by the different stimulation patterns
was determined by placing the thermal probe on the surface of OB slices
at the center of the objective field. Each pattern was repeated 10 times
(interstimulus time: 10 s) and the median normalized temperature trace
was used to calculate the temporal profile of temperature variation for
each pattern in each slice. This procedure was repeated on 10 different
slices and the comparison between patterns was performed on the average
trace (± 95% confidence interval).
The following experimental procedures 2, 3 and 4 were applied to each
recorded neuron.