Cell flow cytometry.
For testing the efficiency of d2eGFP targeting, flow cytometry was used. HEK293T cells were seeded at 1.8×105 per well in a 24-well plate 18-24 h before transfection, and 1 μg remodeled plasmid PCAG::Cas13d-P2A-d2eGFP-PU6::CasRfx-crRNA was transfected by lipofectamine 8000. FACS was performed at 48 hours post-transfection. All transfection experiments were performed in a way of biological triplicates.