Cell lines, culture, and transfection
HEK293T cells were cultured in complete DMEM high glucose medium (Hyclone) supplemented with 10% heat-inactivated fetal bovine serum and maintained in a humidified atmosphere of 5% CO2 at 37 °C. Cells were transfected with plasmid by Lipofectamine™ 3000 Transfection Reagent (Thermo Fisher, USA) or Lipo8000™ Transfection Reagent (Beyotime, China).