2.5 Chromatography and mass spectrometry
Metabolites were separated by reverse-phase liquid chromatography using
a Waters ACQUITY UPLC HSS T3 column (1.8 µm particle size, 2.1 mm x 100
mm) and subsequent by time-of-flight mass spectrometry on a Waters
Xevo-G2S QTof mass spectrometer. Parameters for liquid chromatography
and mass spectrometer sample acquisition can be found in the
supplementary information (Table S1, S2 ). LC-MS sample
injection order was randomized, and pooled samples were injected every
six sample injections.