2.4 Phenylisothiocyanate and 3-nitrophenylhydrazine
derivatization of serum and urine samples
Derivatization was employed to increase the coverage of the metabolites
captured by metabolomics analysis. Phenylisothiocyanate (PITC) was used
to derivatize amino acids, amino acid derivatives, biogenic amines,
glucose/hexose, lipids, and acylcarnitines, whereas
3-nitrophenylhydrazine (3-NPH) was used to derivatize keto- and
carboxyl-containing compounds. The protocols for PITC and 3-NPH
derivatization were adapted from previously published work by Zheng et.
al.