Authors:
Xiaojun Yin1,†, Carmen H. Li1,2,†(https://orcid.org/0000-0002-6135-2582), Alper
Celik3, Julia E. M. Upton4, 5(https://orcid.org/0000-0001-5320-4232), Christina M.
Ditlof1, 6Ditlof1,6(https://orcid.org/0000-0003-3253-4050),
Jennifer A. Hoang1, 6(https://orcid.org/0000-0001-5320-4232), Alireza
Berenjy1, Akash Kothari1, 2(https://orcid.org/0000-0003-1980-161X), Lisa
Hung1,6 (https://orcid.org/0000-0002-4590-1895),
Thomas Eiwegger1,6,7,8(https://orcid.org/0000-0002-2914-7829)
Affiliations :
1Translational Medicine Program, Research Institute,
Hospital for Sick Children, Toronto, Ontario, Canada
2Institute of Medical Science, Temerty Faculty of
Medicine, University of Toronto, Toronto, Ontario, Canada
3Centre for Computational Medicine, Research
Institute, Hospital for Sick Children, Toronto, Ontario, Canada
4Department of Pediatrics, Temerty Faculty of
Medicine, University of Toronto, Toronto, Ontario, Canada
5Division of Immunology and Allergy, SickKids Food
Allergy and Anaphylaxis Program, Hospital for Sick Children
6Department of Immunology, Temerty Faculty of
Medicine, University of Toronto, Toronto, Ontario, Canada
7Karl Landsteiner University of Health Sciences, Krems
an der Donau, Austria,
8Department of Pediatric and Adolescent Medicine,
University Hospital St. Pölten, St. Pölten, Austria
† Contributed equally
Corresponding Author : Thomas Eiwegger, MD, Department of
Pediatric and Adolescent Medicine, University Hospital St. Pölten,
Dunant-Platz 1, 3100 St. Pölten, Austria. E-mail:
thomas.eiwegger@stpoelten.lknoe.at, Tel.: +43 2742-9004-11740
Conflict of Interest Statement: XY, CHL, AC, CMD, JAH, AB, AK,
and LH have nothing to disclose. JEMU reports grants/research support
from DBV Technologies, Regeneron, CIHR, ALK-Abello, SickKids Food
Allergy and Anaphylaxis Program, Advisory board for Pfizer, Kaleo,
Bausch Health, Food Allergy Canada; in-kind drug donation from Novartis,
other for Astra-Zeneca, all outside the current work. TE reports to act
as local PI for company sponsored trials by DBV and sub-investigator for
Regeneron, holds grants from Innovation Fund Denmark, CIHR outside the
submitted work. He is Co-Investigator or scientific lead in three
investigator-initiated oral immunotherapy trials supported by the Food
Allergy and Anaphylaxis Program SickKids and serves as an associate
editor for Allergy. He/his lab received unconditional/in-kind
contributions from Macro Array Diagnostics and an unrestricted grant
from ALK. He holds advisory board roles for ALK, Nutricia/Danone and
Aimmune. TE reports lecture fees from Novartis.
Funding Information: This work was supported by The Hospital
for Sick Children (Food Allergy and Anaphylaxis Program, start-up funds
by the SickKids Research Institute and the Department of Pediatrics,
Restracomp Graduate Scholarship to CHL, AK, and LH). CHL, AK, LH are the
recipients of the Canadian Institutes of Health Research Fredrick
Banting and Charles Best Canada Graduate Scholarship.
Statement of Author Contribution: XY, CHL, AC, CMD, JAH, and AB
contributed to data acquisition. JEMU and TE contributed to experimental
designs. XY, CHL, AC, AK, LH, JEMU, and TE contributed to data
interpretation and preparation of the manuscript. The final version of
the manuscript was approved by all authors.
Keywords : food allergy, pediatrics, mass cytometry
Abbreviations : CyTOF; cytometry by time-of-flight, OIT; oral
immunotherapy, PMA/Iono; Phorbol 12-myristate 13-acetate-ionomycin,
tSNE; t-Distributed Stochastic Neighbour Embedding
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