RNA extraction
RNA was extracted from host hindgut tissue using TRIzol® reagent (Life
Technologies, Mississauga, ON, CAT=15596018) following the
manufacturer’s protocol. RNA was dissolved in sterile water and treated
with TURBO™ DNase (Life Technologies, Mississauga, ON) to remove genomic
DNA contamination and preserved at –80°C until RNA sequencing or cDNA
synthesis and qPCR were performed (see below).