Figure 1 - The for gene in V. canescens. A) Maximum likelihood phylogenetic tree of the for gene in insects. Sequences used from 39 species of insects: hemiptera (2), orthoptera (2), coleoptera (4), lepidoptera (6), diptera (15), hymenoptera (10). Sequence from V. canescens figured in bold within hymenoptera cluster. One crustacean (Daphnia pulex ) and one mammal (Mus musculus ) sequences were used as outgroups. Phylogeny was constructed from the 533 aminoacids located in the conserved C-terminal region of the protein. Branch support was estimated by likelihood-ration test, aLRT values ≥0.90 were indicated with empty circles, and ≥0.95 with full circles. B) Schematic of the for gene model and 17 associated isoforms identified in V. canescens. The for gene located on the scaffold 64 of V. canescens genome was constituted of 13 exons (dark blue boxes). The two conserved functional protein domains (two cGMP binding domains and protein kinase domain) were located at the 3’ extremity of the gene sequence and were represented under the genome sequence (in blue and red respectively). The 5’ part of the gene exhibited variations, with 4 distinct promotors identified (pr1-4 , dark blue arrows), and the corresponding transcripts were labelled accordingly (Vcan27706 to Vcan27709 ). A total of 17 isoforms were identified within RNA-seq libraries issued from sexual and asexual V. canescens heads, and were all represented under the genome sequence with coding sequences figured in dark grey boxes and non-coding sequences (5’ and 3’ UTRs) in pale grey boxes. The amplicon used to quantify for transcript with RT-qPCR was common to almost all isoforms (excepted Vcan_27707i12 ) and figured in green.