4. Conclusions
The whole-cell catalysis of primary diols by G. oxydans with different methylene-numbers were significantly different in products. Primary diols with C≤4 will only be oxidized to the hydroxyl acids, while primary diols with C≥5 will be further oxidized to diacids. Importantly, the catalysis of C5 diols with the pH control successfully selective and directionality produce hydroxyl acids without any diacids, and the output is the highest. In conclusion, the whole-cell catalysis of G. oxydans for hydroxyl acids bio-production was established for industrialization. Moreover, these findings provided valuable insights for promoting environmentally friendly industrial production of hydroxyl acids (C2-C6).