Abstract:
Hydroxyl
acid has become an important chemical in the field of materials and
medicine due to its dual functional modules. Fortunately,Gluconobacter oxydans whole-cell catalysis is on spotlight with
promising potential in bio-catalyzing polyhydroxy chemical to produce
hydroxyl acids. Therefore, straight-chain primary diols (C2-C6) were
investigated as substrates oxidized by G. oxydans .
As
results, we found a fantastic critical point of methylene-number
determining end-products. G. oxydans catalyzes C4 and smaller
methylene-number compounds only forming hydroxyl acids, but C5/C6 can be
converted to diacids. Furthermore, it was important that we successfully
selective and directionality controlled the product of C5/C6 primary
diols to hydroxyl acids instead of diacids through the regulation of
pH≥5.5. Finally, we successfully produced nearly 102.3 g/L
5‑hydroxyvaleric acid during 48 h with 99.8% yield by sealed-oxygen
supply (SOS) biotechnology which is the highest level. These findings
have important reference significance for the selective and
directionality bioconversion of primary diols into hydroxyl acids and
provide a promising path for the industrial development of hydroxyl
acids with integrating C2-C6.
Keywords:
Hydroxyl acid; Ultra-high titer; Selective and directionality;
Whole-cell catalysis;Gluconobacter oxydans