4. Conclusions
The whole-cell catalysis of primary diols by G. oxydans with
different methylene-numbers were significantly different in products.
Primary diols with C≤4 will only be oxidized to the hydroxyl acids,
while primary diols with C≥5 will be further oxidized to diacids.
Importantly, the catalysis of C5 diols with the pH control successfully
selective and directionality produce hydroxyl acids without any diacids,
and the output is the highest. In conclusion, the whole-cell catalysis
of G. oxydans for hydroxyl acids bio-production was established
for industrialization. Moreover, these findings provided valuable
insights for promoting environmentally friendly industrial production of
hydroxyl acids (C2-C6).