Ca modulates cell structure and division
During plant growth and development, cell structure remodeling and cell
division are the primary processes, which involve the regulation of a
series of cell wall and cytoskeleton related
events.62,63Our study showed exogenous Ca supply influenced the expression of some
cell wall and cytoskeleton related proteins (Table1).
Alpha-1,4-glucan-protein synthase 1 (spot 68) have a role in the cell
wall polysaccharides biosynthesis
process.64 Xylose
isomerase (spot 69) participates in the synthesis of xylose, which is an
important structure component of cell
wall.65 Cinnamyl
alcohol dehydrogenase (spot 8) is the enzyme catalyzing the last step of
lignin biosynthesis and is of vital importance to cell wall
formation.66 In the
present study, the abundance of pinoresinol-lariciresinol reductase
(PLR, spot 7), a member of the
pinoresinol-lariciresinol/isoflavone/phenylcoumaran benzylic ether
reductase (PIP) family, that catalyzes two early steps in lignan
biosynthesis67, was
found to be increased under high Ca treatment (Table 1). Together, The
up-regulation of the above four proteins under high Ca treatment suggest
sufficient exogenous Ca potentially accelerate the biosynthesis and
accumulation of substrates for cell wall assembly, and further
facilitate P. massoniana seedling growth and development.
In plants, the actin cytoskeleton system is of vital significance to
cell development and
morphogenesis.68 The
down-regulated actin 2 at low Ca level implying Ca deficiency suppressed
the expression of actin 2, and resulted in distributed P.
massoniana cell division and development. Under medium or high Ca
level, increased expression of actin 2 was observed compared with low Ca
level, suggesting cell actin cytoskeleton system is likely recovered.
Collectively, enough exogenous Ca supply is indispensible for P.
massoniana cell structure reshaping and cell division in the process of
plant growth and development.