Experiment design and workflow
The purpose of this study is to explore the effects and the potential molecular mechanisms of different soil Ca supplies on P. massoniana growth and physiology. The overall experimental design and workflow is descripted as follow. Briefly, after soil Ca leaching and soil nutrient recovery, the P. massoniana seedlings were transplanted into the prepared soil containing high Ca (H), medium Ca (M) or low Ca (L). Medium Ca level was used as the control group; low or high Ca level were used as treatment groups, which simulated Ca deficiency condition or Ca adequacy condition, respectively. After two months cultivation, the growth and development phenotype were observed and photographed. The fresh leaf tissues were harvested for physiological index measurement. A comparative proteomics analysis was performed with the collected leaves to discovery the DEPs under various Ca treatments. Various bioinformatics analyses were implemented to systematically interpret these DEPs. The qRT-PCR analyses for several Ca related genes were performed to reveal the mechanism at transcription level.