Proteome response of P. massoniana seedling leaves to various Ca levels
To illustrate the potential mechanisms underlying Ca regulated response of P. massoniana , a comparative proteomics analysis for the leaves collected from the seedlings cultivated at various Ca levels was performed. As a result, approximate 1000 protein spots were reproducibly resolved on each gel. In total, 74 DEPs were identified among various Ca cultivated seedlings by 2.0 fold and p < 0.05 (Figure 2A and Table 1). As shown in Supplementary Figure S1, Compared with medium Ca group, low Ca induced 25 DEPs (16 up-regulated and 9 down-regulated) and high Ca resulted in 62 DEPs (46 up-regulated and 16 down-regulated). In the comparison between low Ca group and high Ca group, 65 DEPs were found (44 up-regulated and 21 down-regulated). A close-up view of several representative DEP spots was shown in Figure 2B.