Protein hierarchical clustering analysis for DEPs
A hierarchical clustering was carried out to gain a systematic overview
of the expression dynamics of the 74 DEPs resulted from different Ca
level treatments. In this analysis, proteins showed similar expression
patterns were grouped together. As shown in Figure 4, approximate
two-thirds of the 74 DEPs were down-regulated or remain stable under low
Ca treatment. While, the expression abundances of these proteins
increased under high Ca application. These proteins mainly belong to
photosynthesis, energy production, material nitrogen, transcription and
signaling, and heat shock proteins. The expression dynamics of these
proteins suggested Ca deficiency probable distributed these biological
processes while adequate exogenous Ca supply facilitated these
processes. We also observed that some proteins showed stable or even
increased abundance under low Ca condition, while displayed
significantly reduced abundance in high Ca level environment. The
numbers of this group included a variety of redox homeostasis related
proteins and chaperonin proteins.