Experiment design and workflow
The purpose of this study is to explore the effects and the potential
molecular mechanisms of different soil Ca supplies on P.
massoniana growth and physiology. The overall experimental design and
workflow is descripted as follow. Briefly, after soil Ca leaching and
soil nutrient recovery, the P. massoniana seedlings were
transplanted into the prepared soil containing high Ca (H), medium Ca
(M) or low Ca (L). Medium Ca level was used as the control group; low or
high Ca level were used as treatment groups, which simulated Ca
deficiency condition or Ca adequacy condition, respectively. After two
months cultivation, the growth and development phenotype were observed
and photographed. The fresh leaf tissues were harvested for
physiological index measurement. A comparative proteomics analysis was
performed with the collected leaves to discovery the DEPs under various
Ca treatments. Various bioinformatics analyses were implemented to
systematically interpret these DEPs. The qRT-PCR analyses for several Ca
related genes were performed to reveal the mechanism at transcription
level.