Statistical Analysis
The Wilcoxon rank-sum test was used to analyze clinical characteristics between the groups. Alpha diversity was indicated by the Shannon index, Chao1 index and Simpson index, through the Wilcoxon rank-sum test. The β-diversity index compared between groups were directly presented using the Adonis nonparametric test. The Spearman correlation test was used to assess the relevance between clinical indicators and ASVs.
RESULTS
1. Clinical parameters of polycystic ovary syndrome and control participants
We summarized the baseline characteristics of each group of participants (Table 1). Compared with the control group, the PCOS group had higher levels of BMI, LH, E2, AMH and T (P<0.05), while the highest levels of AMH, LH, and total testosterone was found in the PA group (P<0.05). As for the lipid metabolism profile, the levels of low-density lipoprotein (LDL), triglycerides (TG) and total cholesterol (TC) were significantly higher in the PCOS group than in the control group (P<0.05) (Supplementary Table 1).