Limitations
A random forest model was conducted to classify PCOS, which showed the
high accuracy of bacteria biomarkers for PCOS patients in Ji Nan
(AUC=0.8), which indicated the significant association between PCOS and
aberrant VMB. The ability of our model to distinguish PCOS has not been
verified in other populations.
We excluded clinical symptoms, such as abnormal leucorrhea, pruritus
vulva, and vaginal congestion at the time of sampling. Only asymptomatic
case-control differences were observed and the VMB difference between
symptomatic PCOS and controls was not yet determined. Therefore, we lack
studies on symptomatic PCOS patients.