Functional verification of PIM1
To validate the function of PIM1, 4 male tree sparrows from LJX were
dosed daily by oral gavage at 100 mg/kg of SGI-1776, an experimental
PIM1 kinase small molecule inhibitor, and the same numbers of LJX males
were used as control group. After 1 week’s treatment, we analyzed the
sperm traits of two groups. However, we find there is no observed sperm
traits variation between two groups. The sperm length and velocity of
PIM1 inhibitor treatment group show no difference with those of the
control group and the natural LJX population (Figure 3C). We then use
the transcriptome data to quantify PIM1 expression level, and we
find that the PIM1 expression level of treatment group is similar
to control group.