The number of
PD-L1+/IAIE+/MerTK+intrahepatic MPs is lower in TAF-treated CDAHF diet-fed NASH mice
We performed flow cytometry analysis after mouse liver digestion to
confirm the role of intrahepatic MPs role in the NASH mouse model using
the liver of CDAHF diet-fed mouse treated with mock or TAF. A
representative gating strategy for intrahepatic recruited
(CD11bhigh F4/80low) and resident
(CD11blow F4/80high) MPs is shown in
Fig 3A. The TAF-treated CDAHF diet-fed group showed considerably
decreased intrahepatic recruited (CD11bhighF4/80low) MPs than the mock-treated one. However,
there were no significant differences in resident
(CD11blow F4/80high) MPs between the
TAF-treated CDAHF group and mock-treated one (Fig 2B). The intrahepatic
IAIE+ and MerTK+ MP MFI values were
significantly lower in the TAF-treated CDAHF diet group than in the
mock-treated one (Fig 3C). The intrahepatic
PD-L1+/IAIE+/MerTK+-positive
MP number was remarkably decreased in the TAF-treated HFHC diet group
(Fig 3D). Furthermore, the number of CD11b+F4/80+ MPs per g liver weight was significantly
reduced in the TAF-treated CDAHF diet group than in the mock-treated one
(Supplementary Fig S2C).