The number of PD-L1+/IAIE+/MerTK+intrahepatic MPs is lower in TAF-treated CDAHF diet-fed NASH mice
We performed flow cytometry analysis after mouse liver digestion to confirm the role of intrahepatic MPs role in the NASH mouse model using the liver of CDAHF diet-fed mouse treated with mock or TAF. A representative gating strategy for intrahepatic recruited (CD11bhigh F4/80low) and resident (CD11blow F4/80high) MPs is shown in Fig 3A. The TAF-treated CDAHF diet-fed group showed considerably decreased intrahepatic recruited (CD11bhighF4/80low) MPs than the mock-treated one. However, there were no significant differences in resident (CD11blow F4/80high) MPs between the TAF-treated CDAHF group and mock-treated one (Fig 2B). The intrahepatic IAIE+ and MerTK+ MP MFI values were significantly lower in the TAF-treated CDAHF diet group than in the mock-treated one (Fig 3C). The intrahepatic PD-L1+/IAIE+/MerTK+-positive MP number was remarkably decreased in the TAF-treated HFHC diet group (Fig 3D). Furthermore, the number of CD11b+F4/80+ MPs per g liver weight was significantly reduced in the TAF-treated CDAHF diet group than in the mock-treated one (Supplementary Fig S2C).