Accessory gland size changes during recovery
Measurements were done on glands dissected from virgin males, as a previous mating and the accompanying transfer of seminal fluid proteins reduces AG size (Ruhmann et al., 2016). We dissected glands from males 20-24 hours, two, four and six days old and followed procedures as carried out in (Ruhmann et al., 2016). We also measured wing length as an indicator of male body size to control for the allometric relationship between both factors (Shingleton et al., 2007). We measured both AGs and wings for each male and the mean was used for the analysis. In total, the AGs and wings of 15-26 males per treatment and day were measured.