Maturation of the male reproductive system during
recovery
In order to gain insights into the mechanistic basis of the recovery
process we measured changes in male accessory gland (AG) size during the
recovery process. We further checked for the presence of sperm in the
seminal vesicles (SVs) and measured SV size and sperm viability to test
the effects of elevated developmental temperatures on sperm production
and quality. All observations were done under a microscope (ZEISS,
AxioVision Software) with an Olympus SC50 5-megapixel color microscope
camera; at a 50X amplification. Further measurements (measurements of
the organ areas, wing length and sperm head counts) were carried out
with ImageJ (Wayne Rasband).