Declarations:
Ethics approval and consent to participate: Not applicable
Consent for publication: Not applicable
Availability of data and materials : The sequencing datasets
supporting the conclusions of this article are available under the NCBI
SRA: SUB11545621. Additional Data including GCxGC data can be found in
Supplemental Materials file.
Competing interests : The authors declare no conflict of interest.
The funders had no role in the design of the study, in the collection,
analyses, or interpretation of data; in the writing of the manuscript,
or in the decision to publish the results.
Funding: This project was financially supported by the Pioneer
Oil Company (Vincennes, IN) and the Purdue Enhanced Oil Recovery Lab.
Author Contributions: ETH: Conceptualization, Formal analysis,
Data curation, Investigation, Methodology, Project administration,
Supervision, Validation, Visualization; Roles/Writing - original draft.
LECM: Formal analysis, Methodology, Data curation, Investigation,
Writing - review & editing. GK: Funding acquisition, Project
administration, Resources, Writing - review & editing. KVS:
Conceptualization, Funding acquisition, Project administration,
Resources, Supervision, Writing - review & editing. All authors have
read and agreed to the published version of the manuscript.
Acknowledgments: We would like to thank the members of Purdue’s
Enhance Oil Recovery Lab, specifically Dr. Nate Schultheiss, Dr. Thomas
Everett, Dr. Jeremy Holtsclaw, Dr. Katherine Wehde, and Timothy
Henderson, who made this work possible by coordinating well samplings,
provided experimental feedback, and supplying technical supported and
insight for our work. We would also like to thank Dr. Stephen Lindemann
and his lab at Purdue Food Science for providing access to their Micro
GC for H2S analyses. We are also grateful to the Purdue
University Genomics Core for their role in sequencing the 16S rRNA gene
amplicons and the Purdue Institute of Inflammation, Immunology, and
Infectious Disease for access to the Bell computing cluster which
allowed us to analyze the sequencing reads. Additionally, we are
thankful to both Bindley Bioscience Center and the Fuel Laboratory of
Renewable Energy at Purdue University for the research space and support
staff where this research was carried out.