Summary of molecular findings
Of the 1,370,873 reads retained following DADA2 and additional
filtering, 1,059,644 reads were not prey reads; this reduction came
largely from sequences of the spiders themselves (679,534 or 64.1%
Philodromidae reads) followed by fungi (234,630 reads, 22.1%).
Parasitoid wasps represented an additional 60,060 (5.7%) of the
non-prey reads (see S3 in Supplemental Information). Following prey read
curation, there was an average of 2,288.4 ± 288.1 reads and 2.55 ± 0.13
ASVs per individual when combining across loci. 16s returned the highest
number of ASVs at 110 ASVs, followed by 28s at 44 ASVs and 18s at 10
ASVs. This was reflected in the number of specimens retained following
filtering procedures as well; 112 specimens with 16s reads, 76 specimens
with 28s reads and 17 specimens with 18s reads.