Determination of Methyl Esters of Fatty Acids by Gas Chromatography (FAME)
The analysis of FAME of samples was determined using gas chromatography system (HP 6890, Agilent Technologies, DE, USA) equipped with flame ionization detector (FID) described by International Olive Council Methods (modified)–COI/ T.20/ Doc. No.33 2015 (IOC, 2017). DB 23,30m*0.25 mm*0.250 µm capillary column (AgilentJ&WGC Columns, USA) was used for analyses. The injection volume was 1 µl and the temperature of the detector and injector was 250°C. The temperature of the oven was programmed from 170°C to 210°C in increments of 2°C/min. The analysis was terminated by keeping the temperature at 210°C for 10 minutes.