Determination of Sterol Composition and Amount by Capillary
Column Gas Chromatography
The analysis of sterol composition was made according to the methods in
the Turkish Food Codex (Anonymous, 2014). Analysis was performed using
Gas Chromatography (Agilent Technologies 6850, USA) equipped with flame
ionization detector (FID) using a 30 mx0.25mmx0.25µm Supelco 24034
column. The injection volume was 1 µl and the temperature of the
detector was 290°C and injector was 280°C. The temperature of the oven
was programmed from 260°C. Hydrojen was used as carrier gas at an
injection volume of 0.5 mL/min. The flow rate was 0.7/0.8 mL/min. The
split ratio was 1:50. It was silylated using Pyridine and BSTFA+TMCS as
the silylation reagent. 5α-cholestan-3β-ol was used as internal standard
in the analysis. Results were given in both % and mg/kg.