Determination of Sterol Composition and Amount by Capillary Column Gas Chromatography
The analysis of sterol composition was made according to the methods in the Turkish Food Codex (Anonymous, 2014). Analysis was performed using Gas Chromatography (Agilent Technologies 6850, USA) equipped with flame ionization detector (FID) using a 30 mx0.25mmx0.25µm Supelco 24034 column. The injection volume was 1 µl and the temperature of the detector was 290°C and injector was 280°C. The temperature of the oven was programmed from 260°C. Hydrojen was used as carrier gas at an injection volume of 0.5 mL/min. The flow rate was 0.7/0.8 mL/min. The split ratio was 1:50. It was silylated using Pyridine and BSTFA+TMCS as the silylation reagent. 5α-cholestan-3β-ol was used as internal standard in the analysis. Results were given in both % and mg/kg.