Determination of Methyl Esters of Fatty Acids by Gas
Chromatography (FAME)
The analysis of FAME of samples was determined using gas chromatography
system (HP 6890, Agilent Technologies, DE, USA) equipped with flame
ionization detector (FID) described by International Olive Council
Methods (modified)–COI/ T.20/ Doc. No.33 2015 (IOC, 2017). DB
23,30m*0.25 mm*0.250 µm capillary column (AgilentJ&WGC Columns, USA)
was used for analyses. The injection volume was 1 µl and the temperature
of the detector and injector was 250°C. The temperature of the oven was
programmed from 170°C to 210°C in increments of 2°C/min. The analysis
was terminated by keeping the temperature at 210°C for 10 minutes.