HPV genotyping
We used Hologic Aptima HPV test for testing 14 types hrHPV E6/E7 mRNA (types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68) and Hybribio Rapid GenoArray to determine the 21 specific HPV types including 13 high-risk types (types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 68), 6 low-risk types (types 6, 11, 42, 43, 44, and CP8304 [81]) and 2 probable high-risk types (types 53 and 66). The tests were carried out and reported according to manufacturer’s instructions. It should be noted that we included patients who had completed primary cervical cancer screening not only in our hospital but also in other hospitals, therefore, the HPV testing kits were various (mainly Roche Cobas 4800 HPV test and Qiagen Digene HC2 HPV DNA test). As such, the HPV testing could not be uniformed. The HPV results were recorded as hrHPV+ and hrHPV-.