Data Analysis
Graphing and statistical analysis was performed with GraphPad Prism (Version 9). All data sets were checked for normality using D’Agostino & Pearson test. Details of statistical tests, significance and sample sizes are reported in the appropriate figure legends. All data plotted represent mean ± SEM. For western blot experiments, statistical differences between groups were determined by unpaired t test or Mann-Whitney test. Statistical significance of confocal imaging data was evaluated by Mann-Whitney test. For electrophysiological recordings: Normalized peak currents were analyzed by Kruskal-Wallis test followed by the Dunn’s post hoc test; the significance of the I-V curves was analyzed by multiple Mann-Whitney tests; peak current density as well asV1/2 midpoint potential and k slope factor were compared using Mann-Whitney test. For resting membrane potential and rheobase, the significance was analyzed by Mann-Whitney test; the significance of the number of evoked action potentials per step was analyzed by multiple Mann-Whitney tests. Behavioral data was analyzed by multiple Mann-Whitney tests and Mann-Whitney test for time-course experiments and area under the curve respectively. Detailed statistical analyses are presented in Table S1 .