Figure 6. CBD3063 does not inhibit other voltage-gated calcium
channels . (A, C, E, G) Double Boltzmann fits for L-, P/Q-. R-, and
T-type current density–voltage curves respectively. Multiple
Mann-Whitney tests. (B, D, F, H) Summary of bar graph showing peak L-,
P/Q-. R-, and T-type calcium current densities (pA/pF). p value
as indicated; Mann-Whitney test. N=7-11 cells; Error bars indicate mean
± SEM; Half-maximal activation potential (V1/2 )
and slope values (k ) for activation and inactivation are
presented in Table 2 .
As CRMP2 has been reported to regulate the functional activity of
Na+ channels (Dustrude, Moutal, Yang, Wang, Khanna &
Khanna, 2016; Dustrude, Perez-Miller, Francois-Moutal, Moutal, Khanna &
Khanna, 2017; Dustrude, Wilson, Ju, Xiao & Khanna, 2013), we
additionally tested if CBD3063 could affect Na+currents in sensory neurons. CDB3063 did not affect
Na+ current density (DMSO: -455.7 ± 78.70 pA/pF;
CBD3063: -455.0 ± 60.79 pA/pF) or gating properties of
Na+ channels in DRG neurons (Figure S5 andTable 2 ). Furthermore, we found that CBD3063 had no effect on
voltage-gated potassium channels (DMSO: 420.9 ± 54.19 pA/pF; CBD3063:
422.1 ± 52.70 pA/pF; Figure S6 ). Altogether, the above results
demonstrate that CBD3063 specifically inhibits CaV2.2,
sparing all other voltage-gated ion channels expressed in DRG sensory
neurons.