CBD3063 decreases excitability of rat sensory neurons
Cav2.2 channels have critical roles in controlling the excitability of DRG neurons, according to earlier studies (Yang et al., 2018). Since Cav2.2 populations are affected by CBD3063, we next investigated whether CBD3063 may alter the excitability of DRG sensory neurons (Figure 7 ). Action potentials (AP) were elicited in rat sensory neurons during whole cell current-clamp tests in response to current injections from 0-120 pA after overnight incubation with 0.1% DMSO or 20 µM CBD3063. Representative traces are shown inFigure 7A. While the resting membrane potential remained unchanged between these two conditions (DMSO: -44.50 ± 1.478 mV; CBD3063: -50.00 ± 1.915 mV; Figure 7B ), CBD3063 significantly increased the rheobase – the minimum current necessary to evoke an AP – compared to the control (DMSO: 15.00 ± 3.416 mV; CBD3063: 48.33 ± 9.098 mV; Figure 7C ). CBD3063 also reduced the number of evoked APs over current injection steps ranging from 10 to 120 pA (Figure 7D) . These results show that CBD3063 mediated inhibition of the Cav2.2 channels culminates to curb sensory neuron excitability.