Methods:
First, changes in DNAm and gene expression were detected with multiple
targeted bisulfite enrichment sequencing (MethTarget) and qPCR in
peripheral blood of 51 healthy controls (HCs) and 55 asthmatic patients.
Secondly, the correlation between the DNAm levels of specific altered
CpG sites and the pulmonary function indicators of asthma patients was
evaluated. Lastly, the Receiver Operator Characteristic (ROC) curve and
Principal Component Analysis (PCA) were used to identify the feasibility
of the candidate CpG sites as biomarkers for asthma.