Genome acquisition of complete polyprotein gene
Three MuKV-positive samples were randomly selected to amplify the
full-length polyprotein gene sequences using ten pairs of primers
designed in this study on the Benchling website
(https://benchling.com), referred
to the unpublished sequences in the Genbank database (accession number:
MF352432.1). The primer sequences were shown in Table 1. After
sequencing, there full-length polyprotein genomes were assembled using
Lasergene SeqMan software (DNASTAR, Inc. USA, Wisconsin, Madison), and
available in Genbank under the accession numbers MW292480-MW292482.