Genome acquisition of complete polyprotein gene
Three MuKV-positive samples were randomly selected to amplify the full-length polyprotein gene sequences using ten pairs of primers designed in this study on the Benchling website (https://benchling.com), referred to the unpublished sequences in the Genbank database (accession number: MF352432.1). The primer sequences were shown in Table 1. After sequencing, there full-length polyprotein genomes were assembled using Lasergene SeqMan software (DNASTAR, Inc. USA, Wisconsin, Madison), and available in Genbank under the accession numbers MW292480-MW292482.