Selection of SNPs and Genotyping
A total of 86 patients treated with 110 mg DE twice daily were recruited for this study. Peripheral blood samples (3 mL) were collected in EDTA anticoagulant tubes for each patient. Genomic DNA was extracted using a Genomic DNA Purification Kit according to standard protocols. Genotyping of each polymorphism in the ABCB1 and CES1 genes was carried out by the MassARRAY® MALDI-TOF System.
Five SNPs in 2 genes (ABCB1 and CES1) were selected from the National Center for Biotechnology Information (NCBI) database (Table 2). All of the SNPs satisfied the following criteria: (1) SNPs that are associated with cardiac arrhythmia occurrence or development according to the results of existing research; (2) SNPs that may influence the function or expression of ABCB1 and CES1 by NCBI; and (3) minor allele frequency (MAF)≥5% in the Chinese Han population.