2.2 Blood sampling and analytical assays
Blood samples were collected in the morning before dosing when voriconazole reached steady-state. Voriconazole Cminwere analyzed by enzyme immunoassay technique (EMIT)using Siemens VIVA-E automatic detector (Siemens healthcare Diagnostic Inc, Newark, USA) with the voriconazole test kit (No.20190401) from Zhuhai Li zhu Reagent Co., Ltd. The kit has a linear range from 0.5~16 mg/L, possessing good precision (coefficient of variation ≤10%) and accuracy (relative bias within ±15%).