Y: Yes, N:No
DNA extraction, SNP detection by pyrosequencing
Genomic DNA was extracted from whole blood samples using the PureLink
Genomic DNA kit (Invitrogen) according to manufacturer’s protocol.
Genotyping of SNPs at promoter region of HLA-G and CpG41 ofHLA-DRB1 was performed with Pyrosequencing methodology (AQ and
SQA assays), according to manufacturer’s protocols. PCR and sequencing
primers used are presented in Table 2.