2.1 Ethical statement
All animal care and experimental procedures complied with the Animal Research: Reporting of In Vivo Experiments guidelines (Sert, Hurst et al., 2020) and the recommendations of the British Journal of Pharmacology (Lilley, Stanford et al., 2020). Male Sprague-Dawley rats (RRID: RGD_70508) weighing 200–230 g were used as subjects at the beginning of the test. Four were housed per cage (470 mm length×312 mm width×260 mm height) under environmentally controlled conditions (22°C, 12h dark/12h light cycle, and relative humidity of 60%) with access to standard laboratory chow and water ad libitum. The animals were randomly allocated and acclimated for seven days before the experiments. All experimental procedures were performed in accordance with the Care and Use of Laboratory Animals guidelines (National Institutes of Health). The protocols were approved by the Animal Research Ethics Board of Zhejiang University.