2.10 Data analyses
The data and statistical analyses complied with the experimental design and analysis recommendations in pharmacology (Curtis, Alexander et al., 2018). The sample size for statistical analysis was at least five animals per group. The data were analysed by researchers who were blinded to the origin of the data. The declared group size was the number of independent samples, and statistical analysis was performed using these independent values. No data were excluded from the statistical analysis. For western blot analysis, each sample was run in triplicate; the average value of the control group was depicted as “1”, the other groups were normalised to the fold of the control mean. The Shapiro–Wilk normality test was used to assess the normality of the data. The effect of fingolimod treatment on escape latency in the Morris water maze was analysed using two-way repeated-measures analysis of variance (ANOVA). For non-parametric data, the Kruskal-Wallis test was used, followed by Dunn’s post hoc test. All other comparisons were performed using one-way ANOVA followed by Dunnett’s post hoc test. Post hoc tests were conducted only if the Kruskal-Wallis test or F in ANOVA achieved the necessary level of statistical significance and no significant variance in homogeneity. The data are expressed as the mean ± standard error of the mean and were analysed using Prism 8 (GraphPad Software Inc., San Diego, CA, USA; RRID: SCR_002798). Differences were considered statistically significant at p<.05.