2.1 Ethical statement
All animal care and experimental procedures complied with the Animal
Research: Reporting of In Vivo Experiments guidelines
(Sert, Hurst et al., 2020) and the
recommendations of the British Journal of Pharmacology
(Lilley, Stanford et al., 2020). Male
Sprague-Dawley rats (RRID: RGD_70508) weighing 200–230 g were used as
subjects at the beginning of the test. Four were housed per cage (470 mm
length×312 mm width×260 mm height) under environmentally controlled
conditions (22°C, 12h dark/12h light cycle, and relative humidity of
60%) with access to standard laboratory chow and water ad libitum. The
animals were randomly allocated and acclimated for seven days before the
experiments. All experimental procedures were performed in accordance
with the Care and Use of Laboratory Animals guidelines (National
Institutes of Health). The protocols were approved by the Animal
Research Ethics Board of Zhejiang University.