2.9 Cell counts and quantification
A modified unbiased stereology protocol was used for BrdU, DCX, Iba-1, and GFAP quantification (Malberg, Eisch et al., 2000). One out of every six adjacent sections throughout the hippocampus was chosen and processed for immunohistochemistry. The number of immunopositive cells in the dentate gyrus layer and hilus throughout the entire hippocampus was imaged using a Zeiss Axio Imager A2 microscope (Carl Zeiss, Jena, Germany, RRID: SCR_018876) or Pannoramic 250 Flash digital scanners (3D HISTECH Ltd, Budapest, Hungary). The results were expressed as the average number of positive cells per section and analysed using Image-Pro Plus 6.0 software (Media Cybernetics, Silver Spring, USA, RRID: SCR_007369) by an independent researcher.