2.10 Data analyses
The data and statistical analyses complied with the experimental design
and analysis recommendations in pharmacology
(Curtis, Alexander et al., 2018). The
sample size for statistical analysis was at least five animals per
group. The data were analysed by researchers who were blinded to the
origin of the data. The declared group size was the number of
independent samples, and statistical analysis was performed using these
independent values. No data were excluded from the statistical analysis.
For western blot analysis, each sample was run in triplicate; the
average value of the control group was depicted as “1”, the other
groups were normalised to the fold of the control mean. The
Shapiro–Wilk normality test was used to assess the normality of the
data. The effect of fingolimod treatment on escape latency in the Morris
water maze was analysed using two-way repeated-measures analysis of
variance (ANOVA). For non-parametric data, the Kruskal-Wallis test was
used, followed by Dunn’s post hoc test. All other comparisons were
performed using one-way ANOVA followed by Dunnett’s post hoc test. Post
hoc tests were conducted only if the Kruskal-Wallis test or F in ANOVA
achieved the necessary level of statistical significance and no
significant variance in homogeneity. The data are expressed as the mean
± standard error of the mean and were analysed using Prism 8 (GraphPad
Software Inc., San Diego, CA, USA; RRID: SCR_002798). Differences were
considered statistically significant at p<.05.