2.9 Cell counts and quantification
A modified unbiased stereology protocol was used for BrdU, DCX, Iba-1,
and GFAP quantification (Malberg, Eisch et
al., 2000). One out of every six adjacent sections throughout the
hippocampus was chosen and processed for immunohistochemistry. The
number of immunopositive cells in the dentate gyrus layer and hilus
throughout the entire hippocampus was imaged using a Zeiss Axio Imager
A2 microscope (Carl Zeiss, Jena, Germany, RRID: SCR_018876) or
Pannoramic 250 Flash digital scanners (3D HISTECH Ltd, Budapest,
Hungary). The results were expressed as the average number of positive
cells per section and analysed using Image-Pro Plus 6.0 software (Media
Cybernetics, Silver Spring, USA, RRID: SCR_007369) by an independent
researcher.