miRNA |
Known effect of miRNAs on various mammalian
cells |
Predicted/validated glycosylation gene targets in
miRWalk2.0 database and their possible impact on N-glycosylation in
human cells |
miRNA engineering in this study |
Reference |
miR-26b
|
Regulate cell proliferation and apoptosis
|
Gmds (8/12), Fpgt (6/12): regulate biosynthesis of GDP-fucose, and thus
fucosylation
|
cgr-miR-26b OV
|
(43-46) &
miRwalk 2.0
|
miR-30a
|
Regulate cell growth and productivity in CHO cells
|
B4galt1(8/12), SLC35A5(6/12),
SLC35A3(6/12), SLC35C1(8/12).
|
cgr-miR-30a OV
|
(47, 48) &
miRwalk 2.0
|
miR-30b
|
Regulate cell growth and productivity in CHO cells and changing
apoptosis, O-glycosylation in human cancer cells
|
Fut8 (9/12): regulate fucosylation;
Gale(7/12), B4galt1(8/12): regulate galactosylation;
SLC35A2(7/12): regulate transport of UDP-galactose and thus
galactosylation;
SLC35A3(7/12): regulate transport of UDP-GlcNAc, and thus antennarity;
SLC35A4(8/12), SLC35A5(6/12): ): regulate transport of both
UDP-galactose and UDP-GlcNAc, and thus galactosylation and antennarity;
SLC35C1(8/12): regulate transport of GDP-fucose, and thus
fucosylation
|
cgr-miR-30b OV
|
(47-50) &
miRwalk 2.0
|
miR-34a
|
Regulate cell proliferation and apoptosis
|
Mgat1 (7/12), Mgat4a (10/12), Mgat4b (6/12) , Mgat5b (8/12): regulate
antennarity;
Fut8 (10/12): regulate fucosylation; B4galt1(8/12): regulate
galactosylation
|
cgr-miR-34a OV
|
(51-54) &
miRwalk 2.0
|
miR-34c
|
Regulate cell proliferation apoptosis, and DNA replication
|
Mgat1 (8/12), Mgat4a (10/12): regulate antennarity;
Fut8 (9/12): regulate fucosylation
St3gal1(8/12): regulate sialylation
|
cgr-miR-34c KO
|
(55-60) &
miRwalk 2.0
|
miR-122
|
|
Mgat4a (9/12): regulate antennarity
Fut8 (8/12): regulate fucosylation
|
cgr-miR-122 OV
|
miRwalk 2.0
|
miR-128
|
Regulate cell proliferation, apoptosis, self-renewal, and
differentiation
|
Mgat1 (7/12), Mgat4b (8/12): regulate antennarity
|
cgr-miR-128 KO
|
(61-65) &
miRwalk 2.0
|
miR-181a
|
Regulate cell proliferation, apoptosis and differentiation
|
B4galt1(9/12): regulate galactosylation
|
cgr-miR-181a and b
KO in combination
|
(66-77) &
miRwalk 2.0
|
miR-181b
|
|
St3gal1(8/12) : regulate sialylation
B4galt1(9/12): regulate galactosylation
|
|
|
miR449a
|
Regulate cell proliferation and apoptosis
|
Mgat4a (10/12): regulate antennarity
Fut8 (10/12): regulate fucosylation
|
putative-cgr-miR449a OV
|
(78-81) &
miRwalk 2.0
|