5 Conclusion
We proposed a new approach to making small diameter vessels with
complicated patterns and special cell distribution. The two-step
crosslinking method created a continuous connection between different
printed parts and resulted an intact tubular structure with biological
advantages, as well proper sealing and mechanical properties. We
accomplished this method by the investigation of GelMA properties varied
by crosslinking time and optimized by crosslinking process. We
fabricated a small vessel that possesses printed outer layer of SMCs and
perfused inner layer of HUVECs by GelMA bioink printing and two-step
crosslinking process. We also observed that the SMCs and HUVECs were
expanded, proliferated, and functionalized during post printing culture.
Our study might provide a new fabrication method for the construction of
small diameter vessel with clinically relevant size and characteristics.