Soybean Oil Purification
Purification of soybean oil was performed according to the chromatographic method developed by Yoshida et al. (1992) with a chromatographic glass column (36 × 3.4 cm I.D.) packed with aluminum oxide 60 (activated for 3 h at 200 °C) with a ratio of 1(oil) to 1 (absorbent). As a final step, the amount of tocopherols (Wong et al., 1988), total phenols (Capannesi et al., 2000), and hydroperoxides were determined to ensure the purification process efficiency. The purified soybean oil triacylglycerols were contained inconsiderable values of PV (< 1 meq/kg), phenols and tocopherols (< 1 mg/kg).