Partition Coefficient (log P)
The antioxidants solution in 1-octanol (3 mM) was stored for one hour at 60 °C. Then, the maximum solution absorbance was determined immediately by the UV spectrum (A0). Five milliliters of acetate buffer (0.1 M, pH 5.5) was added to 5 mL of the above solutions and vortexed for 1 min. Finally, the maximum absorbance of the octanol layer (Ax) was measured after 30 min (Gordon et al., 2001). Partition coefficient was calculated from Eq. (3):
\(\log P=\log\left(\frac{A_{X}}{A_{0}-\ A_{X}}\right)\) (3)