Water Content Analysis
The water content changes in the oil samples during lipid peroxidation were determined by Karl Fischer titrator (684 KF Coulometer, Metrohm, Germany). The quantitative determination of the water content of the sample is based on the fact that a given amount of substance I2 leads to the conversion of an equivalent amount of substance H2O in the vicinity of methanol and sulfur dioxide. Therefore, by determining the amount of I2consumed in the reaction, the amount of H2O is determined electrochemically. Briefly, the oil samples (0.1 g) were placed into a cell containing a mixture of chloroform/methanol 70:30 and a reagent of Karl Fischer (CombiCoulomat fritless) (Felgner et al., 2008).