3.4 Functional predictions with PICRUSt2
A total of 11 level-2 pathways that associated with key metabolic functions were contained in the result of functional predictions using PICRUSt2 analysis. Among these, amino acid metabolism, xenobiotics biodegradation and metabolism, lipid metabolism, metabolism of terpenoids and polyketides in female were significantly higher than male (P< 0.05), and glycan biosynthesis and metabolism was significantly lower than male (P < 0.05; Figure 5).