Western blot analysis
Lysates of HepG2 cells (which were treated with 5-Aza-2-DC and
tacrolimus) were run on 8% sodium dodecyl sulphate-polyacrylamide gels,
transferred to polyvinylidene difluoride membranes, and incubated with P
glycoprotein (Abcam Inc. Cambridge, MA). Antibody binding was detected
using enhanced chemiluminescence ECL Plus western blotting detection
reagents (Merck Millipore Ltd. Tullagreen, Carrigtwohill, Co. Cork,
IRELAND).