2.3 Annotation and comparative analysis of the O. xuefengensis
mitogenome
Ab initio prediction was used to generate gene models for the O.
xuefengensis mitogenome. Gene models were identified using MFannot
(https://megasun.bch.umontreal.ca/cgi-bin/dev_mfa/mfannotInterface.pl).
Gene models were then compared against the non-redundant (NR) NCBI
database using BLASTp, in addition to comparison against the SwissProt,
KEGG, and COG databases to establish functional annotation, also using
BLASTp. tRNAs were identified using the tRNAscan-SE program (v1.23,
http://lowelab.ucsc.edu/tRNAscan-SE) and
rRNAs were identified using the
RNAmmer program (v1.2,http://www.cbs.dtu.dk/services/RNAmmer/).
Circular maps of the four mitochondrial genomes were drawn using OGDRAW
(https://chlorobox.mpimp-golm.mpg.de/OGDraw.htmlonline)
[41].