22 Mitochondrial DNA sequencing and genome assembly
About 8 μg of purified DNA was sequenced using a combination of
third-generation sequencing technologies including PacBio RS and
Illumina sequencing platforms. The Illumina data was used to evaluate
the complexity of the genome and correct the PacBio long reads. The raw
paired-end reads were trimmed, and quality controlled using Trimmomatic
with parameters including SLIDINGWINDOW: 4:15 MINLEN: 75 (version 0.36http://www.usadellab.org/cms/uploads/supplementary/Trimmomatic).
Clean data obtained from the above quality control procedures were then
used for further analyses.