Animals
All experiments were performed in accordance with the ethical guidelines set out by the International Association for the Study of Pain and the relevant European legislation (Directive 2010/63/EU) and given approval by the local ethics committee (CEMEA Auvergne; reference 6295). Eight-week-old male C57BL/6j mice were purchased from Janvier Laboratories (Le Genest-Saint-Isle, France). Cav3.2 knock-out (Cav3.2 KO) male mice, originally generated by Chen et al. (2003), and their wild type (WT) littermates were bred in the animal facility of Clermont-Auvergne University. We also used Cav3.2-GFP-flox knock-in (Cav3.2GFP-flox KI) mice, generated by Francois et al. (2015). A conditional Cav3.2 KO mouse in C-LTMRs was generated by crossing the Cav3.2GFP-flox KI mice with Nav1.8cre KI mice (Stirling et al., 2005; François et al., 2015). The resulting mice were designated Cav3.2Nav1.8 cKO, their control littermates (Cav3.2GFP-flox KI mice) were also used. Chimeric mice were generated to determine the role in inflammation of Cav3.2 located in immune versus neuronal cells. All animals were housed under standard laboratory conditions (12-hour light/dark cycle, temperature of 21 to 22°C, 55% humidity under specific pathogen free conditions). Food and water were availablead libitum . They were acclimatized for a week before testing.