Figure 2 Genetic evidence for the functional involvement of
Cav3.2 channels in the development of inflammation and
related pain
Cav3.2 knock out mice (Cav3.2 KO) and
their wild type littermates (WT) received an intraplantar administration
of carrageenan (right ) or an intraarticular injection of CFA
(left ). Mechanical allodynia (A ) and hyperalgesia
(B ) were assessed by the von Frey test using 0.04 g and 1.4 g
filaments, respectively. Edema size (C ) was measured with a
caliper. IL-6 levels in edema (D ) was analyzed by ELISA in
supernatant from culture of edema. Statistical differences were assessed
by two-way ANOVA (Time, Genotype) followed by Bonferroni post-hoc test
[significant differences between WT and Cav3.2 KO
group are indicated by asterisks (*p < 0.05; **p< 0.01; ***p < 0.001)]. Statistical
differences for IL-6 levels in edema were assessed by Mann-Whitney test
[significant differences between WT and Cav3.2 KO
group are indicated by asterisks (*p < 0.05;
***p < 0.001)]. Data represent mean ± SEM.