2.2 Data collection and outcome
Venous plasma hemoglobin (Hb), hematocrit (HCT), mean corpuscular volume (MCV), mean platelet volume (MPV), platelet distribution width (PDW), platelet (PLT), C reactive protein (CRP), creatinine (Cr), serum albumin (ALB), N-terminal pro-natriuretic peptide (NT-proBNP), troponin, uric acid (UA), thyroid-stimulating hormone (TSH), and RDW levels were evaluated using standard biochemical approaches in our clinical laboratory department. Two researchers blinded to patient RDW and clinical outcomes independently analyzed all echocardiographic examinations and measured left atrial diameter (LAD), maximum left ventricular wall thickness (MLVWT), left ventricular end-diastolic diameter (LVEDD), and interventricular septum diameter (IVSD). LVM was measured using the Devereux equation [20]. LVM = 0.8(1.04((IVST + LVEDD + PWT)3 LVEDD3)) + 0.6 g, with LVMI being determined via dividing LVM by body surface area. LVEF was assessed via the biplane Simpson method [21]. Dynamic left ventricular outflow tract obstruction (LVOTO) was assessed via continuous-wave Doppler and estimated with the simplified Bernoulli equation [22].