2.2 Data collection and outcome
Venous plasma hemoglobin (Hb), hematocrit (HCT), mean corpuscular volume
(MCV), mean platelet volume (MPV), platelet distribution width (PDW),
platelet (PLT), C reactive protein (CRP), creatinine (Cr), serum albumin
(ALB), N-terminal pro-natriuretic peptide (NT-proBNP), troponin, uric
acid (UA), thyroid-stimulating hormone (TSH), and RDW levels were
evaluated using standard biochemical approaches in our clinical
laboratory department. Two researchers blinded to patient RDW and
clinical outcomes independently analyzed all echocardiographic
examinations and measured left atrial diameter (LAD), maximum left
ventricular wall thickness (MLVWT), left ventricular end-diastolic
diameter (LVEDD), and interventricular septum diameter (IVSD). LVM was
measured using the Devereux equation [20]. LVM = 0.8(1.04((IVST +
LVEDD + PWT)3 LVEDD3)) + 0.6 g, with LVMI being determined via dividing
LVM by body surface area. LVEF was assessed via the biplane Simpson
method [21]. Dynamic left ventricular outflow tract obstruction
(LVOTO) was assessed via continuous-wave Doppler and estimated with the
simplified Bernoulli equation [22].