FIGURE LEGEND
Fig.1. (A) MRI shows enhancing diffusion restricted fairly
large well defined T2/Flair heterogeneously hyperintense mass in left
parietal lobe with effacement of adjacent sulci and gyri and mild
midline shift. (B) Histology demonstrated neuropils surrounded
by small blue round cell tumors in diffuse sheets and rosettes
surrounded by single layer of small round blue cells. (C)Synaptophysin staining of sheets of small round blue cells. Other IHC
stains demonstrated (not shown) GFAP patchy +, NeuN patchy +, Olig2 -,
Chromogranin –, LIN28 –, INI-1 retained, Desmin -, EMA -. Ki67 was
~20%. (D) Unsupervised clustering (tSNE plot;
Supplement: Methods) showed that the tumor grouped closest with ETMR
(red arrow). (E) Copy number aberrations plot derived from
methylation profiling. (F) Focused analysis (using the conumee
package; Supplement: Methods) revealed no structural variation at C19MC
region. (G) The MGMT promoter was methylated.
[Abbreviations in figure : FLAIR: Fluid-attenuated
inversion recovery. DWI: Diffusion weighted imaging. MB:
Medulloblastoma. EPN: Ependymoma. PF: Posterior fossa. HGG: High-grade
glioma. GBM: Glioblastoma. MES: Mesenchymal. MID: Midline. K27M: Diffuse
midline glioma, K27-altered. G34R: Diffuse hemispheric glioma,
G34-mutant. PXA: Pleomorphic xanthoastrocytoma. Ana-PA: Anaplastic
pilocytic astrocytoma. ATRT: Atypical teratoid rhabdoid tumor. ETMR:
Embryonal tumor with multilayered rosettes. HGNET: High-grade
neuroepithelial tumor. NB: Neuroblastoma. EWS: Ewing sarcoma. EFT: Ewing
family tumor. MGMT: O(6)-Methylguanine-DNA-methyltransferase.]