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\begin{document}

\title{Shining light on multi-drug resistant Candida auris: ultraviolet-C
disinfection, wavelength sensitivity, and prevention of biofilm
formation of an emerging yeast pathogen}



\author[1]{Richard Mariita}%
\author[1]{James  Davis}%
\author[2]{Michelle  Lottridge}%
\author[1]{Rajul Randive}%
\affil[1]{Crystal IS Inc., an Asahi Kasei company}%
\affil[2]{Crystal IS Inc., an Asahi Kasei company }%


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  \date{June 18, 2024}


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\begin{abstract}
Background: Candida auris is an emerging fungal pathogen of worldwide
interest. It is associated with high mortality rates and exhibits
increased resistance to antifungals. Ultraviolet-C (UVC) light can be
used to disinfect surfaces to mitigate its spread. In this study, we
analyzed the performance of different UVC wavelengths against C. auris
to determine its wavelength sensitivity and UVC dose requirements and
evaluated biofilm prevention dose requirements on most used materials in
healthcare settings. Objectives: 1. To investigate UVC disinfection
performances and wavelength sensitivity of C. auris; 2. To evaluate the
UVC dose required for prevention of biofilm prevention on stainless
steel. Methods: C. auris was grown following standard procedures. The
study utilized six different UVC LED arrays with wavelengths between 252
and 280 nm. Arrays were set at similar intensities, to obtain doses of
5-40 mJcm-2 and similar irradiation time. Disinfection performance for
each array was determined using log reduction value (LRV) and percentage
reduction by comparing the controls against the irradiated treatments.
Evaluation of the ability of 267 nm UVC LEDs to prevent C. auris biofilm
formation was investigated using stainless steel, plastic coupons, and
poly-cotton fabric. Results: Peak sensitivity to UVC disinfection was
between 267 and 270 nm. With 20 mJcm-2, the study obtained LRV 3. On
steel coupons, 30 mJcm-2 was sufficient to prevent biofilm formation, on
plastic only 10 mJcm-2. A dose of 60 mJcm-2 reduced biofilms on
poly-cotton fabric significantly. Conclusions: Results revealed that C.
auris was most susceptible at 267-270 nm. Additional highlights from the
study allow for the design and implementation of disinfection systems.%
\end{abstract}%



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\verb`Candida_auris_MYA-5001_manuscript_.docx`  available at \url{https://authorea.com/users/442926/articles/543038-shining-light-on-multi-drug-resistant-candida-auris-ultraviolet-c-disinfection-wavelength-sensitivity-and-prevention-of-biofilm-formation-of-an-emerging-yeast-pathogen}

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