Fatty acid composition and iodine value
Fatty acid methyl esters (FAME) synthesized from silflower oil were
separated using an Agilent Technologies (Palo Alto, CA) 6890N GC fitted
with a flame ionization detector and a Supelco (Belefonte, PA) SP2380 30
m × 0.25 mm column. The peaks were identified using a standard mix of
saturated FAME (C8 to C30) and mono- and poly-unsaturated C14, C16, C18,
C20, C22 and C24 (Nu-Check Prep, Elysian, MN). The details of the FAME
preparation and GC parameters are available elsewhere (Chakraborty et
al., 2018). The iodine value (IV) of the oil was calculated from the
fatty acid composition according to AOCS Cd1-85 (AOCS, 2017).