Figure
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Figure 1. Summary of study, from left to right, Gambusia
affinis were collected from warm- (pink) and ambient-source (blue)
populations and taken into the laboratory where they were acclimated at
20 or 30°C for four months. After acclimation, fish behavioural
(boldness, activity) and metabolic traits (standard, routine, maximum
metabolic rates) were measured and compared.
Figure 2. Relationship between fish mass and A) standard
metabolic rate (SMR), B) routine metabolic rate (RMR), and C) maximum
metabolic rate (MMR) in Gambusia affinis . Dashed lines and open
circles represent acclimation at 20°C, and solid lines and filled
circles represent acclimation at 30°C. Warm and ambient refer to the
population’s thermal history (see Table 1). We fit data with simple
linear regression models and denoted significance of these models as:
<0.0001 ’***’, <0.001 ’**’, n = 198/ trait.
Figure 3. Temperature sensitivity of metabolism across
laboratory acclimated ambient- and warm-source populations ofGambusia affinis . We show temperature sensitives for each
measured metabolic trait separately. Data are averages ± 1 SE; n= 8.
Figure 4. Relationship between thermal history and behaviour as
A) boldness (i.e., emergence latency) and B) activity (i.e., time spent
exploring) as population differences. Plots C and D show individual
differences across all populations between mass corrected routine
metabolic rate (RMR), expressed per unit mass, and behaviour as boldness
and activity, respectively. We do not show individuals who did not leave
the refuge, n = 76.