Figure legends
Figure 1. Summary of study, from left to right, Gambusia affinis were collected from warm- (pink) and ambient-source (blue) populations and taken into the laboratory where they were acclimated at 20 or 30°C for four months. After acclimation, fish behavioural (boldness, activity) and metabolic traits (standard, routine, maximum metabolic rates) were measured and compared.
Figure 2. Relationship between fish mass and A) standard metabolic rate (SMR), B) routine metabolic rate (RMR), and C) maximum metabolic rate (MMR) in Gambusia affinis . Dashed lines and open circles represent acclimation at 20°C, and solid lines and filled circles represent acclimation at 30°C. Warm and ambient refer to the population’s thermal history (see Table 1). We fit data with simple linear regression models and denoted significance of these models as: <0.0001 ’***’, <0.001 ’**’, n = 198/ trait.
Figure 3. Temperature sensitivity of metabolism across laboratory acclimated ambient- and warm-source populations ofGambusia affinis . We show temperature sensitives for each measured metabolic trait separately. Data are averages ± 1 SE; n= 8.
Figure 4. Relationship between thermal history and behaviour as A) boldness (i.e., emergence latency) and B) activity (i.e., time spent exploring) as population differences. Plots C and D show individual differences across all populations between mass corrected routine metabolic rate (RMR), expressed per unit mass, and behaviour as boldness and activity, respectively. We do not show individuals who did not leave the refuge, n = 76.