2.1 Patient and sample grouping
Twenty-five children diagnosed with rhinosinusitis and requiring
endoscopic sinus surgery were included in this study from January 2021
to December 2021 in the Department of Otolaryngology Head and Neck
Surgery at our hospital. According to EPOS 2020, 8 patients were
diagnosed with ABRS with orbital complications, and 17 were diagnosed
with Chronic Rhinosinusitis with Nasal Polyps (CRSwNP). None of the
patients had CF, congenital disease, or paranasal sinus masses. The
children with ABRS were treated with anti-infection and comprehensive
therapy before operation, but their symptoms did not improve
significantly. The CRS patients received regular drug treatment in the
outpatient department for 3 months, but their symptoms did not improve
significantly. All the children were admitted to hospital for endoscopic
sinus surgery and nasal secretions were collected for bacterial culture
during surgery, and whole blood, nasal mucosa and polyp samples were
collected for follow-up experiments. Whole blood samples were collected
from parents of some children. All patients’ guardians denied that they
had CF-related diseases or had relatives diagnosed as CF in their
families.