2.3 Quantitative real-time PCR
The expression of CFTR mRNA was measured by qRT-PCR in all polyps and
mucosa. Fluorescent quantitative PCR was performed using a non-specific
fluorescent staining method (SYBR Green I) using Lamboride Taq SYBR
Green Qpcr Remix (R0202). The primer sequence was
CFTR:“F:AGCTGTCAAGCCGTGTTCTAGATA; R:
ATGAGGAGTGCCACTTGCAAA”,β-actin:“F:TTCTACAATGAGCTGCGTGTG;R:
AGAGGCGTACAGGGATAGCA”.