2.3 Quantitative real-time PCR
The expression of CFTR mRNA was measured by qRT-PCR in all polyps and mucosa. Fluorescent quantitative PCR was performed using a non-specific fluorescent staining method (SYBR Green I) using Lamboride Taq SYBR Green Qpcr Remix (R0202). The primer sequence was CFTR:“F:AGCTGTCAAGCCGTGTTCTAGATA; R: ATGAGGAGTGCCACTTGCAAA”,β-actin:“F:TTCTACAATGAGCTGCGTGTG;R: AGAGGCGTACAGGGATAGCA”.