Patterns of synonymous codon usage for Hsp60 genes
To understand the peculiarities of codon usage, it is necessary to
assess not only the degree of codon usage bias, but also the
representation of synonymous codons in a gene, or, in other words, the
patterns of synonymous codon usage. The approach widely used for this
purpose is the calculation of synonymous codon usage bias, reflecting
the peculiarities of amino acid coding, when some codons in a gene are
used more often than other synonymous codons68. Under
the influence of the mutational pressure and natural
selection69, the representation of various codons in a
gene plays an important role in the processes of its
transcription70 and translation71.
Determination of patterns of synonymous codon usage is necessary for
understanding the features of gene/genome evolution. Thus, the relative
synonymous codon usage (RSCU) of 64 codons in the Hsp60 genes was
calculated (Figure 11; Supplementary, RSCU).