Figure 10. Symmetric matrix of p-values of the t-test between the ENC values of the Hsp60 genes from 17 phyla. The statistically indistinguishable ENC values of Hsp60 genes of the two phyla, having a t-test with a p-value greater than 0.05, are marked in black. Statistically different ENC values of the Hsp60 genes of the two phyla, having a t-test with a p-value less than 0.05, are marked in white. The black frames and the “Cluster” X-axis represent the four clusters and their numbers (Roman numerals). Clustering was carried out using the UPGMA algorithm.
According to the results of the t-test (Supplementary, ENC (t-test results)), the ENC values of Hsp60 genes of 14 phyla (cluster I) are statistically indistinguishable (Figure 10). In turn, the ENC values for Actinobacteria (cluster II), Streptophyta (cluster III), and Proteobacteria (cluster IV) are statistically different from all other phyla. It can be assumed that the expression level of Hsp60 genes from Proteobacteria and Actinobacteria is high (34.8 and 31.6, respectively), while the expression level of the Hsp60 genes from Streptophyta is close to low (51.6). It should be noted that more accurate conclusions about the level of Hsp60 gene expression should be made based on the results obtained by conventional laboratory methods.