2.7 RNA extractions
RNA extractions of individual 50µl sample aliquots were performed using the Applied Biosystems MagMax-96 Viral RNA extraction kit (AMB1836-5, Thermofisher) following the manufacturer’s protocol for manual extraction methods (MAN0017826). Linear polyacrylamide was used as a carrier in place of carrier RNA. Reactions were eluted into 50µl elution buffer and stored in 96-well plates at -80oC.