Frankia DNA extraction from root nodules and soils
The collected nodule and soil samples were transported to a laboratory
in a cool box, and then we immediately extracted Frankia DNA. The
root nodules were sterilized using 10% (v/v) Clorox bleach. One lobe
was picked from each washed nodule, sliced using sterilized razor
blades, and crushed using sterilized homogenization sticks. Bacterial
DNA extraction from the crushed lobes and 300 mg of the soil samples
from alder rhizosphere and riparian soils was performed using a DNeasy
Blood & Tissue Kit (Qiagen) and NucleoSpin Soil (Macherey-Nagel),
respectively.