Enzyme-linked Immunosorbent assay for IL-6, TNF-α, IFN-γ, IL-17
and IL-22
The cytokine levels in the serum were collected and allowed to clot at 4
°C for 2 hours. The clots were removed by centrifuging at 7000 rpm for
30 min in a refrigerated centrifuge. Serum was obtained from all
subjects by centrifugation and stored at-80°C for determination of
cytokines levels. The levels of cytokines in the serum/supernatant were
measured by Enzyme-linked Immunosorbent Assay (ELISA) using commercially
available IL-6, TNF-α, IFN-γ, IL-17 and IL-22 ELISA kits (BOSTER, Wuhan,
China) according to the manufacturer’s instructions.