2.3 | Sequencing and phylogenetic analysis
VP1 sequences were generated using previously described methods (Knowles
et al., 2016). Complete VP1 nucleotide sequences were aligned using
BioEdit 7.0.5.3 (Hall, 1999) and Clustal W 1.83 (Thompson et al., 1994).
Optimal nucleotide substitution models were computed for each serotype
using MEGA 7 (Kumar et al., 2016). The maximum likelihood algorithm was
used to construct phylogenetic trees employing using MEGA 7. One
thousand bootstrap pseudo-replicates were used to assess branching
confidence.