Figure 5. RNA-Seq analysis of PF IL-17RE+ Th17 cells and IL-17RE- Th17 cells. (A) Gating and sorting of PF CCR6+CCR4+IL-17RE+ Th17 cells and CCR6+CCR4+IL-17RE- Th17 cells. (B) Volcano plot displaying the DETs. (C) Cluster analysis of DETs. 17RE-: IL-17RE- Th17. 17RE+: IL-17RE+ Th17. (D to F) Enrichment of up-regulated DETs for GO terms in the category of biological process terms (D), cellular component terms (E), and molecular function terms (F). (G) Enrichment of up-regulated DETs for KEGG terms.
Figure 6. OXPHOS and ETC in PF IL-17RE+ Th17 cells and IL-17RE- Th17 cells. (A) Heat map of top 40 metabolism-related DETs (FDR < 0.05). (B) Validation of DETs in (A) by qRT-PCR. N=5~7 per group. The data are presented as min to max. (C) ATP levels in IL-17RE+ Th17 cells and IL-17RE-Th17 cells. N=6 per group. (D) ROS levels measured by H2DCFDA staining. N=5 per group. *: p<0.05; **: p<0.01; ***: p<0.001. Mann-Whitney U test.