Figure 3. Identification of live PF Th17 subsets according to IL-17RE and CD27. (A) Subsets in CD14-CXCR3-CCR10-CD4+ cells based on the expression of CCR6 and CCR4. (B) Relative mRNA levels of indicated master regulators in the subpopulations described in (A). PB: blood CD3+CD4+ T cells as control. R6: CCR6. R4: CCR4. N=3 per group. (C) Flow cytometry plots showing IL-17A expression in the subpopulations described in (A). (D) Frequencies of IL-17A+ cells in the 4 subpopulations. (E) Expression of IL-17RE and CD27 on the surface of the CCR6+CCR4+ subpopulation. (F) Relative mRNA levels of IL-17A in IL-17RE+CD27- cells, IL-17RE+CD27+ cells, and IL-17RE-CD27- cells. (G) Relative mRNA levels of RORγt in the same three subsets as (F). ). *: p<0.05; **: p<0.01; ***: p<0.001. N=5 per group in (D) to (G). Kruskal-Wallis test.