Figure legend
Figure 1 . A , Frequencies of CRTH2+and CRTH2-Tregs in patients with asthma (n = 120) and
healthy controls (n =30). B ,
Frequency of
CRTH2+Tregs in patients with eosinophilic allergic
(EA, n =30), eosinophilic non-allergic (EN, n = 7), non-eosinophilic
allergic (NA, n =69), non-eosinophilic non-allergic (NN, n = 14) and
healthy control (HC, n = 30). C , Frequency of
CRTH2+Tregs compared between allergic and uncontrolled
asthma (asthma control test (ACT) < 20). D ,
Correlation between the frequency of CRTH2+Tregs and
ACT and % predicted peak expiratory flow rate (PEFR) in all asthmatic
patients. E, Frequency of CRTH2+Tregs in
patients with asthma exacerbation (n = 30) and controlled (n = 15)
compared between baseline and after 3 weeks. *p < 0.05,
**p < 0.01, ****p < 0.0001.
Figure 2. A,Representative tSNE plot clustered 6 T cells population,
CRTH2+CD4+CD25-(dark blue), CRTH2+CD8+ (dark red),
CRTH2+Tregs (dark green),
CRTH2-CD4+CD25-(light blue), CRTH2-CD8+ (light red)
and CRTH2-Tregs (light green) of patient with
allergic, non-allergic asthma and healthy control. RelativePTGDR2 (B ), transcription factor genes(FOXP3 andGATA3 ) (C ), cytokine genes (IL10 , IL4 andIL13 ) (D ) expression in Tregs of patients with allergic
(n = 10), non-allergic asthma (n = 10) and healthy controls (n = 10).E, In vitro experiment, after 3 days of
CD4+ T cells culture, 10-5 M
dexamethasone (Dexa) decreased CD69 expression on
CRTH2+Tregs in condition with/without PGD2 stimulation
of asthmatic patients (n = 10). F , Fresh whole blood of
asthmatic patients (n = 10) were pretreated with 1µM ramatoban (DP2
antagonist) and then stimulated with PGD2. The numbers of
CD69+CRTH2+Tregs were investigated 1
hour after PGD2 administration. G , Tight junction of bronchial
epithelial cells of asthmatic patients (n = 10) compared between
unstimulated, PGD2, CRTH2+Tregs with or without PGD2
and CRTH2-Tregs. Transepithelial electrical resistance
(TER) measurements were examined at 0, 1, 6, 24, 48, and 72 hours.H , Levels of IL-4, IL-5 and IL-13 in the supernatant of
bronchial epithelial culture. *p < 0.05, **p< 0.01, ***p < 0.001, ****p< 0.0001.