2.2 | Nucleic acid isolation, library construction and
sequencing
Total genomic DNA was isolated using a sodium dodecyl
sulphate/proteinase K digestion (Gasser et al., 2006) followed by
phenol-chloroform extraction and ethanol precipitation. Genomic DNA was
sheared into 200-800 bp for paired-end libraries preparation according
to the manufacturer’s instructions of the DIPSEQ platform (BGI-Shenzhen,
Shenzhen, China). Libraries were then subjected to the DIPSEQ-T1
sequencer for short-read whole genome sequencing (WGS) sequencing (Table
S1).