2.3.3 Biochemical measurements
A venous blood sample (10 ml) was collected from every patient after
overnight fasting. Plasma were applied to determine insulin, FPG, MDA,
TAC, uric acid, hs-CRP, LPS, and 8-iso-PGF2α. FPG and uric acid were
measured via the enzymatic method by an autoanalyzer using kits
(Pars-Azmoon Co., Tehran, Iran). Insulin concentration was determined
using a chemiluminescent immunoassay method. 8-iso-PGF2 (Abcam,
Cambridge, UK), and LPS levels (LAL kit endpoint-QCL1000; Cambrex
BioScience, Walkersville, Maryland, USA) were determined using an
enzyme-linked immunosorbent assay (ELISA) kit. hs-CRP serum
concentrations were measured using an immunoturbidimetric assay (Pars
Azmoon Co., Tehran, Iran). The activity levels of TAC, GSH-Px, and SOD
were measured through a colorimetric method (TAC: RANDOX kits, GSH-Px:
RANSEL kits and SOD: RANSOD kits; RANDOX Laboratory, UK) by an automatic
analyzer. MDA levels were determined by spectrofluorimeter (Kontron,
model SFM 25A, Italy).26 The method of Aebi was
applied to determine catalase activity.27