2.3 Cell transfection and luciferase assay
HEK-293T cells cultured in 96-well plate were transiently transfected
with NF-κB-responsive luciferase reporter plasmid using Lipofectamine
3000 transfection reagent. The cells were cultured for 24 h and treated
with the compounds together with TNF-α (100 ng/ml) for 6 h. Luciferase
activity was determined using luciferase assay kits according to the
manufacturer’s instructions.