2.1 Materials
Antibodies against IκB kinase β (IKK, #8943), phospho-IKK α/β
(Ser176/180) (p-IKK, #2697), B-cells inhibitor alpha
(IκB-α, #4814), nuclear factor
(NF-κB) (p65, #4764), phospho-NF-κB p65(Ser536) (NF-κB, #3033), The
stress-activated protein kinase/Jun-aminoterminal kinase SAPK/JNK (JNK,
#9252), phosphor-SAPK/JNK (Thr183/Tyr185) (p-JNK,#9251), BiP (#3309),
CHOP (#2895), Sequestosome 1 (p62, #39749), Light Chain 3 A
(LC3A, #4599), and glyceraldehydes-3-phosphate dehydrogenase (GAPDH,
#5174) were purchased from Cell Signaling Technology; ELISA kits used
in measurement of plasma parameters are as follows: Mouse MCP 1 (Boster,
EK0568), Mouse IL-6 (Boster, EK0411) , Mouse IL-1β (Boster, EK0394),
Mouse TNF-α (Boster, EK0527). Lipofectamine 3000 reagent was obtained
from Invitrogen. Parthenolide (PTL) was purchased from MedChem Express
(MCE). Mouse macrophage colony stimulating factor (mM-CSF). Tumor
necrosis factor (TNF)-α lipopolysaccharide (LPS) were obtained from
Sigma Aldrich. Luciferase substrates were purchased from Vazyme.
High-glucose Dulbecco’s modified
eagle’s medium (HG-DMEM) was purchased from Hyclon.