Figure 2. Compound 270 inhibits NF-κB transcriptional activity and directly interacts with NF-κB in HEK 293 and RAW 264.7 cells.
(A) Chemical structure of 269 and 270. (B) The NF-κB transcriptional inhibition activity of 269 and 270. (C) Effect of 270 on NF-κB protein stabilization in HEK 293 cells. HEK 293 cells transfected with NF-κB expression plasmid were treated with 5 μM 270 for 3 h before heated at different temperatures. Protein levels were analyzed by western blotting. (D) Protein band densities shown in (C) were quantified by normalizing to β-actin. (E) Effect of 270 on NF-κB protein stabilization in RAW 264.7 macrophages. RAW 264.7 macrophages were treated with 5 μM 270 for 3 h before heated at different temperatures. Protein levels were analyzed by western blotting. (F) Protein band densities shown in (E) were quantified by normalizing to β-actin. The data are presented as the means ± SEM. All experiments were performed in three replicates. Two-tailed Student’s t-test. *P < 0.05, **P <0.01, ***P < 0.001 versus DMSO.
Figure 3