Figure 6. Compound 270 improves the mice acute kidney injury caused by intraperitoneal injection of LPS through attenuating renal inflammation state
(A) HE staining of kidney sections after 24 h LPS-induced kidney injury. Scale bar = 200 μm (n = 6). (B) The concentrations of plasma Crea and BUN, (n = 6). (C) The relative mRNA level of NGAL and KIM1 in mice kidney tissue and normalized by GAPDH, (n = 6). (D) The relative mRNA level of each pro-inflammatory gene in renal tissues was detected by use of RT-PCR and normalized by GAPDH, (n = 6). (E) Western blot analysis of inflammatory signaling protein expression in kidney tissues, (n = 6). (F) Relative protein expression levels were determined by densitometric quantification of the immunoblots shown in (E), (n = 6). The data are presented as the means ± SEM. One-tailed Student’s t-test. *P < 0.05, **P <0.01, ***P < 0.001 compared with the LPS group.
Figure 7