Statistical Analysis
Statistical analyses were performed using Microsoft Excel (Microsoft,
Redmond, WA, US), Analyse-it (Analyse-it Software Ltd., Leeds, UK) and
MedCalc version 12.7.2.0 (MedCalc Software, Ostend, Belgium). The normal
distribution of the data was determined by the Shapiro-Wilks test. An
independent sample t -test was used to compare two independent
groups, and a one-way ANOVA test was used for comparison of more than
two groups. G-power 3.1 software was used to determine the sample size.
We assigned the effect size as 0.8, alpha error as 5%, and power as
95%. Since we divided our patient group into subgroups, the allocation
ratio was determined as 6.20 The required sample size
was estimated to be 140 in total (20 for group 1 and 120 for group 2).