Evaluation and validation
Final model was evaluated by the minimum OFV and goodness-of-fit plots. A bootstrap with 1,000 replicates was performed to verify the accuracy and stability of the model, and the parameters were summarized as the median and 95% confidence interval (CI). Furthermore, visual predictive checks (VPC) with 1,000 simulations were performed to assess the predictive performance of model. The observed 5th, 50th, and 95th percentiles were plotted with their respective simulated 95% CI.