Materials and methods
Reagents and compounds
Compounds DNFB (MW: 516.45), 2-aminoethoxydiphenyl borate (2-APB), capsaicin (Cap), ruthenium red (RR), GSK1016790A (GSK101), A-967079 (A-96) and allyl isothiocyanate (AITC) were purchased from Sigma-Aldrich (St. Louis, MO). RR were made as stock solutions in before use internal solution. Other compounds were made as stock solutions in DMSO before use. Compounds used for the measurement of intracellular fluorescent calcium were diluted in the Hanks’ balanced salt solution (HBSS). Compounds were diluted in perfusion solution for patch-clamp recordings. DNFB was dissolved in solvent (acetone/olive oil = 4:1) for establishment of DNFB induced inflammation model (Bhol and Schechter, 2005).