Establishment of DNFB induced inflammation model of the dorsal skin in mice
The hair of mice was shaved firstly, and then the remaining hair was removed with depilatory cream. The interval of one day is recorded as the day 0 and is not processed. At day 1, 100 μl 0.5 % DNFB was topically applied to sensitize the prepared dorsal skin of mice. No DNFB treatment at day 2, 3 and 4. At day 5 and day 6, 50 μl 0.2 % DNFB was applied to challenge the skin respectively. The solvent (acetone/olive oil, 4:1) was used as a control.