Genetic Analysis
Blood samples were drawn directly into PAXgene tubes (Qiagen Inc, Valencia, Calif) and immediately frozen and stored at -80 degrees Celsius for mRNA stabilization and extraction, per the manufacturer’s recommendation.
Human transcriptome profiling assay were done with Applied Biosystems™ Clariom™ D Pico Assay, human (ThermoFisher Scientific) according to the manufacturer’s protocol in Brown University Genomics Core Facility for use on the GeneChip™ 3000 instrument system. Transcriptome Analysis Console (TAC) software was used to analyze and visualize global expression patterns of genes, exons, pathways, and alternative splicing events.